D275V : Biochemical assays

The D290V substitution in Saccharomyces cerevisiae Pol2 mimicking D275V in human POLE eliminates the exonuclease activity of DNA polymerase ε.

• Barbari, S.R. et al. Enhanced polymerase activity permits efficient synthesis by cancer-associated DNA polymerase ε variants at low dNTP levels. Nucleic Acids Res 50, 8023-8040 (2022). https://pubmed.ncbi.nlm.nih.gov/35822874/