L606M : Biochemical assays

The L606M substitution in human POLD1 and the analogous L612M substitution in Saccharomyces cerevisiae Pol3 increase the polymerase activity of DNA polymerase δ and decrease the accuracy of DNA synthesis in vitro. Data with yeast L612M variant indicates a defect in polymerase-to-exonuclease active site switching.

• Nick McElhinny, S.A., Stith, C.M., Burgers, P.M.J. & Kunkel, T.A. Inefficient proofreading and biased error rates during inaccurate DNA synthesis by a mutant derivative of Saccharomyces cerevisiae DNA polymerase δ. J Biol Chem 282, 2324–2332 (2007). https://pubmed.ncbi.nlm.nih.gov/17121822/
• Schmitt, M.W., Matsumoto, Y. & Loeb, L.A. High fidelity and lesion bypass capability of human DNA polymerase δ. Biochimie 91, 1163–1172 (2009). https://pubmed.ncbi.nlm.nih.gov/19540301/